Marked enhancement of Acinetobacter sp. organophosphorus hydrolase activity by a single residue substitution Ile211Ala
نویسندگان
چکیده
منابع مشابه
Evaluation of the organophosphorus hydrolase enzyme activity in creams and investigation of its stability
The main purpose of this project is investigation of the organophosphorus hydrolase (OPH) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the OPH enzyme stability in formulated creams. OPH enzyme was extracted and purified from strain flavobacterium. The w/o and o/w creams were prepared using different formulations. In order to achieve an emulsion with m...
متن کاملDetection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase.
As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was mo...
متن کاملA single amino acid substitution converts a carboxylesterase to an organophosphorus hydrolase and confers insecticide resistance on a blowfly.
Resistance to organophosphorus (OP) insecticides is associated with decreased carboxylesterase activity in several insect species. It has been proposed that the resistance may be the result of a mutation in a carboxylesterase that simultaneously reduces its carboxylesterase activity and confers an OP hydrolase activity (the "mutant ali-esterase hypothesis"). In the sheep blowfly, Lucilia cuprin...
متن کاملOrganophosphorus hydrolase multilayer modified microcantilevers for organophosphorus detection.
We report a biosensor based on organophosphorus hydrolase (OPH) multilayer modified microcantilever (MCL) for detection of organophosphorus compounds (OPs). The assay is based on substrate-dependent bending of the OPH functionalized MCLs. The cantilever bending amplitude at equilibrium was a function of the concentration of paraoxon with the dynamic range extending from 10(-7) to 10(-3)M. The l...
متن کاملbioactivity enhancement of hydroxyapatite by silicon substitution
objective: silicon is an effective element in bone biomineralization; hence si-substituted hydroxyapatite can be a relevant bioceramic as bone materials substitution. materials and methods: stoichiometric hydroxyapatite (ha) and si-substituted hydroxyapatite (si-ha) with different contents of si substitution were synthesized successfully by a hydrothermal method using ca(no3)2, (nh4)3po4 or (n...
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ژورنال
عنوان ژورنال: Bioresources and Bioprocessing
سال: 2015
ISSN: 2197-4365
DOI: 10.1186/s40643-015-0067-3